Implemented kill switches: 1
Kill Switch 1
Class
# Format: # Write how you would classify them.
Toxins
| Name | Number | Comment |
|---|---|---|
| parE | Toxin of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE |
Antagonists
| Name | Number | Comment |
|---|---|---|
| ParD | ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE | |
| 3-oxo-hexanoyl-homoserine lactone | BBa_C0061 | HSL inhibits the promotor of the toxin plasmid |
| LuxR | BBa_C0062 | LuxR inhibits the promotor of the toxin plasmid |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| lux pR | BBa_R0062 | Promoter activated by LuxR in concert with HSL |
| luxl | BBa_C0061 | Synthesizes 3OC6HSL which binds to LuxR |
| luxR | BBa_C0062 | In complex with HSL the LuxR binds to the Lux promoter |
| double terminator | BBa_B0015 | Double terminator consisting of BBa_B0010 and BBa_B0012 |
| parD | ParD is the antidote of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE | |
| HSL-mediated luxR repressor | BBa_R0061 | Promoter that is inhibited by HSL and LuxR |
| aiiA | BBa_C0060 | encodes an enzyme that catalyzes the degradation of N-acyl-homoserine lactones/HSL |
| parE | Toxin of the plasmid-encoded toxin-antitoxin (TA) system ParD-ParE |